2YCB
Structure of the archaeal beta-CASP protein with N-terminal KH domains from Methanothermobacter thermautotrophicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.027, 112.027, 400.608 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| R-factor | 0.21538 |
| Rwork | 0.215 |
| R-free | 0.25081 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i7v |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.956 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.120 | 0.600 |
| Number of reflections | 46893 | |
| <I/σ(I)> | 17.9 | 2 |
| Completeness [%] | 99.3 | 93.1 |
| Redundancy | 11 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM KH2PO4 PH 5.2, 2% PEG 2000 MME, 7% ETHYLENE GLYCOL, 0.5 MM ZNCL2, 1 MM TRIS(2- CARBOXYETHYL)PHOSPHINE. |
