2YCA
Mixed-function P450 MycG in complex with mycinamicin III in P21212 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-03-02 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.747, 162.330, 49.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 162.330 - 1.800 |
| R-factor | 0.1551 |
| Rwork | 0.152 |
| R-free | 0.21027 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2Y4H |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.875 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.170 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.120 | 0.930 |
| Number of reflections | 43565 | |
| <I/σ(I)> | 7.6 | 1.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.6 M SUCCINIC ACID, PH 7.0 . |
