2YC2
Intraflagellar Transport Complex 25-27 from Chlamydomonas
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.050, 96.220, 66.970 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 48.110 - 2.588 |
| R-factor | 0.2067 |
| Rwork | 0.204 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1TVG 2o52 1y2k 2AED 1n6h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.335 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 2.590 | 1.590 |
| Rmerge | 0.090 | 0.560 |
| Number of reflections | 16992 | |
| <I/σ(I)> | 13 | 2 |
| Completeness [%] | 97.3 | 85 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 20% PEG 3350, 50MM MES PH 6.0 |
