2YC1
Crystal structure of the human derived single chain antibody fragment (scFv) 9004G in complex with Cn2 toxin from the scorpion Centruroides noxius Hoffmann
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-07-27 |
| Detector | MARRESEARCH |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 219.709, 219.709, 219.709 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.280 - 1.900 |
| R-factor | 0.18743 |
| Rwork | 0.186 |
| R-free | 0.21212 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ybr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.940 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.300 |
| Number of reflections | 68864 | |
| <I/σ(I)> | 6.3 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.5 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 1.4 M SODIUM / POTASSIUM PHOSPHATE PH 5.6 |
