2YB4
Structure of an amidohydrolase from Chromobacterium violaceum (EFI target EFI-500202) with bound SO4, no metal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.070, 46.530, 130.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.775 - 2.200 |
R-factor | 0.1735 |
Rwork | 0.170 |
R-free | 0.23270 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 1.053 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | 0.260 |
Number of reflections | 12953 | |
<I/σ(I)> | 10.3 | 4.8 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.1 | PROTEIN (15 MG/ML IN 10 MM HEPES PH 7.5, 150 MM NACL, 10% GLYCEROL) ADDED TO 25% PEG 3350, 100 MM BIS-TRIS PH 6.1, 200 MM AMSO4. CRYOSOLUTION WAS RESERVOIR WITH 20% ETHYLENE GLYCOL |