2YA1
Product complex of a multi-modular glycogen-degrading pneumococcal virulence factor SpuA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.137, 86.429, 193.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.2082 |
| Rwork | 0.205 |
| R-free | 0.26519 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fh6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.348 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 56573 | |
| <I/σ(I)> | 9.4 | 2.8 |
| Completeness [%] | 92.6 | 94.8 |
| Redundancy | 4.25 | 4.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
