2Y9W

Crystal structure of PPO3, a tyrosinase from Agaricus bisporus, in deoxy-form that contains additional unknown lectin-like subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-02
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 2
Unit cell lengths	104.060, 104.520, 109.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.130 - 2.300
R-factor	0.184
Rwork	0.181
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDE ENTRIES 1JS8 1lnl 1wx2 AND 1BT1
RMSD bond length	0.010
RMSD bond angle	1.164
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.100	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.120	0.550
Number of reflections	53813
<I/σ(I)>	7.7	2.3
Completeness [%]	100.0	100
Redundancy	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		10% PEG 4000, 100 MM NA ACETATE PH 4.6, 5 MM HOLMIUM CHLORIDE.