2Y9R
Crystal structure of the M10 domain of Titin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 42 |
| Unit cell lengths | 43.450, 43.450, 56.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.420 - 1.900 |
| R-factor | 0.19592 |
| Rwork | 0.194 |
| R-free | 0.24108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wp3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.543 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.420 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.550 |
| Number of reflections | 8350 | |
| <I/σ(I)> | 10.8 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 35% PEG 400, 0.1M BIS-TRIS PH 6.5 |
