2Y98
Structure of the mixed-function P450 MycG in complex with mycinamicin IV in P21212 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.285, 162.025, 50.394 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 162.020 - 1.650 |
| R-factor | 0.15075 |
| Rwork | 0.148 |
| R-free | 0.20611 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z36 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.173 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.010 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.630 |
| Number of reflections | 47944 | |
| <I/σ(I)> | 15 | 1.6 |
| Completeness [%] | 84.9 | 40.6 |
| Redundancy | 6.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 296 | 8% PEG 4000, 0.2 M AMMONIUM SULFATE, 0.1 M BISTRIS PH 5.5, TEMP 23 C |
