2Y96
Structure of human dual-specificity phosphatase 27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 126.012, 126.012, 125.758 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.380 |
| R-factor | 0.20257 |
| Rwork | 0.201 |
| R-free | 0.22482 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pq5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.514 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.070 | 0.720 |
| Number of reflections | 24047 | |
| <I/σ(I)> | 48.2 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.1 | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 10 MG/ML PROTEIN, 0.2M AMMONIUM SULFATE, 25% W/V PEG3350, ROOM TEMPERATURE, pH 6 |
