2Y8W
Structure of CRISPR endoribonuclease Cse3 bound to 20 nt RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.100, 66.150, 74.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.362 - 1.800 |
| R-factor | 0.1683 |
| Rwork | 0.166 |
| R-free | 0.21010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wj9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.064 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.260 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.120 | 0.700 |
| Number of reflections | 20275 | |
| <I/σ(I)> | 14.51 | 3.57 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 30% (V/V) PEG 300. |
