2Y8Q

Structure of the regulatory fragment of mammalian AMPK in complex with one ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-01-12
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	49.026, 119.918, 130.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.234
Rwork	0.232
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v8q
RMSD bond length	0.010
RMSD bond angle	1.644
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.040	0.360
Number of reflections	17426
<I/σ(I)>	12.4	2.3
Completeness [%]	93.8	93.5
Redundancy	2.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1