2Y8K

Structure of CtGH5-CBM6, an arabinoxylan-specific xylanase.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	69.120, 75.550, 105.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.580 - 1.470
R-factor	0.14685
Rwork	0.146
R-free	0.16627
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.014
RMSD bond angle	1.448
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXCDE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.740	1.550
High resolution limit [Å]	1.470	1.470
Rmerge	0.080	0.640
Number of reflections	94925
<I/σ(I)>	13.9	2.8
Completeness [%]	99.9	99.9
Redundancy	7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20% PEG 3000, 150 MM SODIUM CITRATE, PH 5.5
1				20% PEG 3000, 150 MM SODIUM CITRATE, PH 5.5