2Y8E
Crystal structure of D. melanogaster Rab6 GTPase bound to GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.520, 42.710, 86.860 |
| Unit cell angles | 90.00, 133.12, 90.00 |
Refinement procedure
| Resolution | 63.400 - 1.390 |
| R-factor | 0.14607 |
| Rwork | 0.145 |
| R-free | 0.17296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yzq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.561 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.700 | 1.470 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.050 | 0.280 |
| Number of reflections | 61395 | |
| <I/σ(I)> | 9.7 | 4.4 |
| Completeness [%] | 97.4 | 95.3 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.4 M AMMONIUM SULPHATE, 0.1 M IMIDAZOLE PH 8.0, 2.5% PEG 400, 2.5% DMSO AND 3% TRIMETHYLAMINE N-OXIDE. |
