2Y7E
Crystal structure of the 3-keto-5-aminohexanoate cleavage enzyme (Kce) from Candidatus Cloacamonas acidaminovorans (tetragonal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-05-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 102.188, 102.188, 101.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.760 - 1.280 |
| R-factor | 0.14465 |
| Rwork | 0.143 |
| R-free | 0.17020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y7d |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 133532 | |
| <I/σ(I)> | 12.4 | 3.3 |
| Completeness [%] | 97.6 | 89.6 |
| Redundancy | 7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG3350, 0.220 M MG FORMATE, 26.4% GLYCEROL, pH 7.0 |
