2Y7A
Crystal structure of unliganded GTB P156L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.730, 78.290, 143.740 |
| Unit cell angles | 90.00, 88.96, 90.00 |
Refinement procedure
| Resolution | 143.760 - 2.060 |
| R-factor | 0.21716 |
| Rwork | 0.216 |
| R-free | 0.24453 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ziz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.984 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 143.760 | 2.160 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.070 | 0.350 |
| Number of reflections | 75523 | |
| <I/σ(I)> | 12.6 | 2.6 |
| Completeness [%] | 93.2 | 77.6 |
| Redundancy | 2.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 5 MM MOPS PH 7, 1 MM MNCL2, 1 MM DTT, 0.1 M AMSO4, 10% (W/V) PEG 3350. |
