2Y78
Crystal structure of BPSS1823, a Mip-like chaperone from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 54.677, 54.677, 119.172 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.646 - 0.910 |
| R-factor | 0.1097 |
| Rwork | 0.109 |
| R-free | 0.11610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1FKB AND 1ROT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.586 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 0.960 |
| High resolution limit [Å] | 0.910 | 0.910 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 125244 | |
| <I/σ(I)> | 13.8 | 2.2 |
| Completeness [%] | 98.3 | 88.2 |
| Redundancy | 5.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1.1 M (NH4)2SO4, 0.05 M BIS-TRIS PH 5.5 |
