2Y6W
Structure of a Bcl-w dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-04 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.227, 76.227, 112.847 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.637 - 2.000 |
R-factor | 0.2011 |
Rwork | 0.199 |
R-free | 0.24420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pq0 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.048 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 1.310 |
Number of reflections | 23159 | |
<I/σ(I)> | 20.5 | 2.2 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 11.8 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 5% (W/V) PEG 3000, 40% (W/V) PEG 400, 0.1M MES PH 6.5. |