2Y6Q
Structure of the TetX monooxygenase in complex with the substrate 7- Iodtetracycline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-23 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.700, 79.960, 88.000 |
| Unit cell angles | 111.10, 90.41, 92.96 |
Refinement procedure
| Resolution | 51.980 - 2.370 |
| R-factor | 0.21937 |
| Rwork | 0.218 |
| R-free | 0.23776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xdo |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.825 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.980 | 2.500 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.090 | 0.490 |
| Number of reflections | 66166 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 93.5 | 69.6 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
