2Y6L
Xylopentaose binding X-2 engineered mutated CBM4-2 Carbohydrate Binding Module from a Thermostable Rhodothermus marinus Xylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-25 |
| Detector | MARRESEARCH MAR165 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.900, 49.480, 62.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.280 |
| R-factor | 0.132 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y6k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.031 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.310 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 38527 | |
| <I/σ(I)> | 15.9 | 3.1 |
| Completeness [%] | 96.9 | 94.9 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.2 M SODIUM CITRATE PH 6.4 |
