2Y6E
Structure of the D1D2 domain of USP4, the conserved catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-06 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 110.500, 151.030, 178.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.590 - 2.400 |
R-factor | 0.1797 |
Rwork | 0.178 |
R-free | 0.21040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gfo |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.600 | 44.600 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.660 |
Number of reflections | 111095 | |
<I/σ(I)> | 11 | 1.4 |
Completeness [%] | 94.9 | 74 |
Redundancy | 3.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | PROTEIN WAS CRYSTALLIZED FROM 100MM BIS-TRIS PROPANE (PH8.5), 25MM NA2SO4, 18% PEG3350, THEN SOAKED IN 25% ETHYLENEGLYCOL |