2Y65
Crystal structure of Drosophila melanogaster kinesin-1 motor domain dimer-tail complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2010-10-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 113.765, 190.714, 145.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.19787 |
| Rwork | 0.195 |
| R-free | 0.25192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y5w |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.785 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.370 |
| Number of reflections | 80517 | |
| <I/σ(I)> | 21.7 | 5.5 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 8.8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 18% POLYETHYLENE GLYCOL-3350, 0.2M POTASSIUM CHLORIDE, 0.1M HEPES SODIUM PH 7.4 |
