2Y5Z
Mixed-function P450 MycG in complex with mycinamicin III in C2221 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-11-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.305, 100.882, 439.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 219.680 - 2.060 |
| R-factor | 0.15339 |
| Rwork | 0.150 |
| R-free | 0.22139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2Y4H |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.826 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.460 | 2.170 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.080 | 0.360 |
| Number of reflections | 69912 | |
| <I/σ(I)> | 9.8 | 1.5 |
| Completeness [%] | 86.3 | 42.3 |
| Redundancy | 4.4 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 296 | 12.5% PEG MME 5000, 6% TACSIMATE, PH 7.0, 2% BENZAMIDINE HYDROCHLORIDE, 0.15 M NACL; TEMPERATURE 23 DEGREES CELSIUS. |
