2Y5Y
Crystal structure of LacY in complex with an affinity inactivator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-22 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.560, 127.840, 189.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.280 - 3.380 |
| R-factor | 0.2872 |
| Rwork | 0.286 |
| R-free | 0.30270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pv7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.280 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.190 | 0.740 |
| Number of reflections | 35055 | |
| <I/σ(I)> | 4.8 | 1.3 |
| Completeness [%] | 97.7 | 94.3 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PH 6 |
