2Y5S
Crystal structure of Burkholderia cenocepacia dihydropteroate synthase complexed with 7,8-dihydropteroate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-08-06 |
| Detector | RIGAKU CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.662, 86.920, 88.795 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.110 - 1.950 |
| R-factor | 0.17742 |
| Rwork | 0.176 |
| R-free | 0.21114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aj2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.232 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 41750 | |
| <I/σ(I)> | 14.4 | 3.1 |
| Completeness [%] | 98.9 | 93.6 |
| Redundancy | 4.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | RESERVOIR: 0.1 M TRIS/HCL PH 8, 10 % PEG 8000, 0.3 M MGCL2 PROTEIN SOLUTIONS: BCDHPS AT 7.5 MG/ML, 2 MM DIHYDROPTEROATE, 50 MM TRIS/HCL PH 7.5, 250 MM NACL. |
