2Y5B
Structure of USP21 in complex with linear diubiquitin-aldehyde
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.870, 102.200, 86.800 |
| Unit cell angles | 90.00, 99.82, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.700 |
| R-factor | 0.21898 |
| Rwork | 0.216 |
| R-free | 0.27859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2HD5 AND 1UBQ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.035 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0098) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.120 | 0.470 |
| Number of reflections | 24840 | |
| <I/σ(I)> | 8.8 | 2.4 |
| Completeness [%] | 91.1 | 86.2 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 15% PEG8000, 0.2 M NH4SO4, pH 7.4 |
