2Y5B
Structure of USP21 in complex with linear diubiquitin-aldehyde
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-09 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.870, 102.200, 86.800 |
Unit cell angles | 90.00, 99.82, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.700 |
R-factor | 0.21898 |
Rwork | 0.216 |
R-free | 0.27859 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2HD5 AND 1UBQ |
RMSD bond length | 0.007 |
RMSD bond angle | 1.035 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0098) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.800 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.120 | 0.470 |
Number of reflections | 24840 | |
<I/σ(I)> | 8.8 | 2.4 |
Completeness [%] | 91.1 | 86.2 |
Redundancy | 2.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 15% PEG8000, 0.2 M NH4SO4, pH 7.4 |