2Y50
Crystal Structure of Collagenase G from Clostridium histolyticum at 2. 80 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-27 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.260, 108.700, 181.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.830 - 2.800 |
| R-factor | 0.23452 |
| Rwork | 0.232 |
| R-free | 0.28159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y3u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.908 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.560 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 28517 | |
| <I/σ(I)> | 12.4 | 2.8 |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | 23% PEG3350, 0.12M TRISODIUM CITRATE PH 8.3 |
