2Y4Y
Structure of a domain from the type IV pilus biogenesis lipoprotein PilP, from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.200, 74.510, 74.180 |
| Unit cell angles | 90.00, 125.80, 90.00 |
Refinement procedure
| Resolution | 32.220 - 1.700 |
| R-factor | 0.181 |
| Rwork | 0.178 |
| R-free | 0.22400 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.298 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CRANK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.290 |
| Number of reflections | 47367 | |
| <I/σ(I)> | 8.9 | 2.6 |
| Completeness [%] | 94.7 | 92.6 |
| Redundancy | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS-HCL PH 8.5, 0.6M ZINC ACETATE |
