2Y4X
Structure of a domain from the type IV pilus biogenesis lipoprotein PilP, from Pseudomonas aeruginosa PA01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU R-AXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 24.240, 87.790, 42.210 |
Unit cell angles | 90.00, 103.77, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.233 |
Rwork | 0.230 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y4y |
RMSD bond length | 0.028 |
RMSD bond angle | 2.321 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.060 | 0.380 |
Number of reflections | 16259 | |
<I/σ(I)> | 9.7 | 2.4 |
Completeness [%] | 86.7 | 85.8 |
Redundancy | 2.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1M HEPES PH 7.5, 8% (V/V) ETHYLENE GLYCOL, 20% (W/V) PEG 10,000 |