Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-04 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 149.500, 149.500, 154.321 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 129.470 - 2.700 |
| R-factor | 0.19279 |
| Rwork | 0.191 |
| R-free | 0.21878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bo7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.601 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.400 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.120 | 0.550 |
| Number of reflections | 47729 | |
| <I/σ(I)> | 9.5 | 2.8 |
| Completeness [%] | 96.8 | 98.8 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 4% V/V ETHYLENE GLYCOL, 0.1 M BIS-TRIS PROPANE, PH 5.5, 0.3 M MAGNESIUM FORMATE |
