2Y4G
Structure of the Tirandamycin-bound FAD-dependent tirandamycin oxidase TamL in P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-12-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.020, 130.400, 134.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 134.540 - 2.030 |
| R-factor | 0.16271 |
| Rwork | 0.159 |
| R-free | 0.23566 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y08 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.894 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 134.540 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.120 | 0.680 |
| Number of reflections | 67342 | |
| <I/σ(I)> | 7.7 | 1.5 |
| Completeness [%] | 91.7 | 63 |
| Redundancy | 4.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 15% PEG 4000, 0.1M MGCL2, 0.1M HEPES 7.5, ADDITIVE 0.1M MGCL2. |
