2Y4D

X-ray crystallographic structure of E. coli apo-EfeB

Replaces:  2WX6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-11
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	83.315, 86.260, 119.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.000
R-factor	0.20589
Rwork	0.203
R-free	0.26483
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gvk
RMSD bond length	0.015
RMSD bond angle	1.420
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.240
Number of reflections	58929
<I/σ(I)>	26.5	8
Completeness [%]	99.7	98.8
Redundancy	10.7	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	291	2.0M AMMONIUM SULFATE, 5% (V/V) PEG 400, 0.1M HEPES PH 7.0, 291K