2Y48
Crystal structure of LSD1-CoREST in complex with a N-terminal SNAIL peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-30 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.170, 181.460, 234.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.770 - 3.000 |
| R-factor | 0.21296 |
| Rwork | 0.212 |
| R-free | 0.24559 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1d |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.493 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.640 |
| Number of reflections | 50937 | |
| <I/σ(I)> | 9.5 | 1.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.2 M SODIUM/POTASSIUM TARTRATE, 100 MM ADA BUFFER PH 6.5 |
