2Y3Y
Holo-Ni(II) HpNikR is a symmetric tetramer containing four canonic square-planar Ni(II) ions at physiological pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 72.970, 72.970, 116.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.980 - 2.390 |
| R-factor | 0.2317 |
| Rwork | 0.229 |
| R-free | 0.27892 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.216 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 2.520 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.070 | 0.640 |
| Number of reflections | 24202 | |
| <I/σ(I)> | 26.8 | 3.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 20% PEG 3350 AND 0.2 M K/NA-TARTRATE, pH 7.3 |
