2Y3W
N-terminal head domain and beginning of coiled coil domain of Danio rerio SAS-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-05-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 79.360, 79.360, 79.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.492 - 1.980 |
| R-factor | 0.2218 |
| Rwork | 0.221 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y3v |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.759 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.490 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.090 | 1.500 |
| Number of reflections | 38948 | |
| <I/σ(I)> | 12.5 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.5 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.2 | 0.1 M CHES PH 9.2, 36% PEG 600, 1MM DTT. |
