2Y3U
Crystal structure of apo collagenase G from Clostridium histolyticum at 2.55 Angstrom resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.960, 109.050, 182.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.550 |
| R-factor | 0.21043 |
| Rwork | 0.208 |
| R-free | 0.25645 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.152 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.320 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.100 | 0.700 |
| Number of reflections | 33182 | |
| <I/σ(I)> | 9.3 | 1.8 |
| Completeness [%] | 88.1 | 84.4 |
| Redundancy | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | 21.5% PEG 3350, 0.225 M TRISODIUM CITRATE PH 8.3. |
