2Y3G
Se-Met form of Cupriavidus metallidurans CH34 CnrXs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.896, 81.809, 93.336 |
| Unit cell angles | 90.00, 90.47, 90.00 |
Refinement procedure
| Resolution | 46.666 - 1.910 |
| R-factor | 0.1899 |
| Rwork | 0.187 |
| R-free | 0.23820 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.893 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.040 | 0.230 |
| Number of reflections | 36868 | |
| <I/σ(I)> | 13.9 | 3.3 |
| Completeness [%] | 99.0 | 95.9 |
| Redundancy | 6.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 16% PEG 2000MME, 15% GLYCEROL., pH 7.5 |
