2Y3B
Co-bound form of Cupriavidus metallidurans CH34 CnrXs
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 33.033, 33.033, 196.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.575 - 1.554 |
| R-factor | 0.1776 |
| Rwork | 0.176 |
| R-free | 0.20380 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.583 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.040 | 1.640 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 16626 | |
| <I/σ(I)> | 12.2 | 2.4 |
| Completeness [%] | 99.1 | 95.2 |
| Redundancy | 7.2 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M TRIS PH8, 1.6M AMMONIUM SULFATE, 15% GLYCEROL. |
