2Y24
STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION BY ERWINIA CHRYSANTHEMI GH5 GLUCURONOXYLANASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-25 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.578, 59.578, 168.296 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.370 - 1.390 |
| R-factor | 0.12206 |
| Rwork | 0.120 |
| R-free | 0.16930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nof |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.973 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.400 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 70207 | |
| <I/σ(I)> | 8.5 | 1.3 |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | CO-CRYSTALLIZATION USING 0.1 M IMIDAZOLE - DL-MALIC ACID BUFFER, PH 7.5 AND 20% PEG 1500 AS PRECIPITANT SOLUTION |
