2Y1P
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, complexed with citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.950, 46.920, 54.850 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 26.283 - 1.820 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.126 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.280 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.070 | 0.680 |
| Number of reflections | 19148 | |
| <I/σ(I)> | 14.19 | 2.02 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.74 | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.3 | pH 3.3 |
