2Y1B
Crystal structure of the E. coli outer membrane lipoprotein RcsF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97791, 1.5 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.714, 55.714, 61.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.250 - 2.000 |
| R-factor | 0.19913 |
| Rwork | 0.196 |
| R-free | 0.25752 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.157 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.530 |
| Number of reflections | 7786 | |
| <I/σ(I)> | 11.5 | 2.7 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | HANGING DROP: PROTEIN 11MG/ML MIXED WITH I3C 20 MM RESERVOIR: 1.8M AMMONIUM SULFATE, 0.1M SODIUM ACETATE PH 4.5, DROP: 1 MICROL AND 1 MICROL. |
