2Y0T
The mechanisms of HAMP-mediated signaling in transmembrane receptors - the A291F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.070, 34.320, 47.650 |
| Unit cell angles | 90.00, 117.79, 90.00 |
Refinement procedure
| Resolution | 19.560 - 1.300 |
| R-factor | 0.17491 |
| Rwork | 0.173 |
| R-free | 0.20855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.762 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.030 | 0.300 |
| Number of reflections | 28953 | |
| <I/σ(I)> | 19.6 | 3.6 |
| Completeness [%] | 94.7 | 96.5 |
| Redundancy | 3.05 | 3.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20 MM MOPS, 100 MM NACL, 20% PEG 3350., pH 6.5 |
