2Y0M
CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN DOSAGE COMPENSATION FACTORS MSL1 AND MOF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-07 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 180.940, 180.940, 80.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.650 - 2.700 |
| R-factor | 0.22477 |
| Rwork | 0.223 |
| R-free | 0.25587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2giv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0085) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.810 |
| Number of reflections | 18543 | |
| <I/σ(I)> | 14.05 | 2.16 |
| Completeness [%] | 99.1 | 98.6 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M SODIUM ACETATE (PH 5), 1.0 M SODIUM FORMATE. |
