2Y0F
STRUCTURE OF GCPE (IspG) FROM THERMUS THERMOPHILUS HB27
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-27 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.500, 130.970, 101.260 |
Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.202 |
Rwork | 0.199 |
R-free | 0.26300 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.018 |
RMSD bond angle | 1.809 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.070 | 0.470 |
Number of reflections | 71563 | |
<I/σ(I)> | 11.8 | 2.2 |
Completeness [%] | 96.0 | 86.6 |
Redundancy | 3.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PROTEIN SOLUTION 30MM TRIS-HCL PH7.5, 0.15M NACL, 12MG/ML PROTEIN. RESERVOIR/PRECIPITANT 47% MPD, 2% TERT-BUTANOL |