2Y09
The cyanobacterial PP2C-like phosphatase tPphA requires three metals in the catalytic center for efficient catalysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.714, 151.678, 82.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.330 - 1.700 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j82 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.330 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.400 |
| Number of reflections | 27138 | |
| <I/σ(I)> | 19.2 | 2.67 |
| Completeness [%] | 99.1 | 97 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG3350 0.2M CACL2 0.1M TRIS-HCL PH 8.0 |
