2XZG
Clathrin Terminal Domain Complexed with Pitstop 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2009-10-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.340, 73.915, 84.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.850 - 1.700 |
| R-factor | 0.16071 |
| Rwork | 0.159 |
| R-free | 0.19973 |
| Structure solution method | MIR |
| Starting model (for MR) | 1c9i |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.865 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.850 | |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.240 |
| Number of reflections | 47297 | |
| <I/σ(I)> | 24.34 | 4.19 |
| Completeness [%] | 95.1 | 1.7 |
| Redundancy | 5.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20% PEG 3350, 150 MM POTASSIUM ACETATE, 0.1 M TRIS, PH 8.0. |
