2XYV
Crystal structure of the nsp16 nsp10 SARS coronavirus complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-11 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.151, 184.800, 129.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.410 - 2.060 |
| R-factor | 0.20205 |
| Rwork | 0.200 |
| R-free | 0.23361 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fyg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.520 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.110 | 0.350 |
| Number of reflections | 51033 | |
| <I/σ(I)> | 7.5 | 3.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 67 MM M CHES, 0.99 M MGCL2-HEXAYDRATE, 33 MM TRIS-HCL, 8.3% (V/V) PEG 8000, FROZEN IN THE PRESENCE OF 15% (V/V) GLYCEROL, pH 8.5 |
