Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 170.500, 170.500, 100.651 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 147.660 - 2.810 |
| R-factor | 0.24782 |
| Rwork | 0.246 |
| R-free | 0.28425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hmi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.345 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.140 | 2.960 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.100 | 1.020 |
| Number of reflections | 41549 | |
| <I/σ(I)> | 10.8 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.8M SODIUM SUCCINATE, PH 7, VAPOUR DIFFUSION, SITTING DROP, TEMPERATURE 277K |
