2XY4
X-RAY STRUCTURE OF ZNUA-WT FROM SALMONELLA ENTERICA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 143.650, 143.650, 30.811 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.710 |
| R-factor | 0.21115 |
| Rwork | 0.209 |
| R-free | 0.24402 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2prs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.226 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 40175 | |
| <I/σ(I)> | 32.19 | 5.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 11.6 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE 2.5 M, HEPES 0.1 M PH 7.5, PEG 400 2% . |
