2XY3
Structure of the Bacillus subtilis prophage dUTPase with dUpNHpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.474, 98.400, 97.993 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.550 |
| R-factor | 0.19705 |
| Rwork | 0.194 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2XX6 (CHAIN A) |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.770 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.600 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.050 | 0.590 |
| Number of reflections | 31651 | |
| <I/σ(I)> | 37 | 2.55 |
| Completeness [%] | 99.8 | 97.9 |
| Redundancy | 7.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 3.4 M 1,6-HEXANEDIOL, 0.1 M TRIS PH 8.5, 0.2M MGCL2. |
